The examination methods for trichomoniasis include the following:
(1) Hanging drop method
The hanging drop method is the simplest method for checking Trichomonas vaginalis, with a positive rate of 80%-90%. Spread the sample on a slide, add one drop of physiological saline, cover with a coverslip, and examine under a 100-200x lens. The flagellum of the protozoan can be seen to move. If 5% neutral red is added to the physiological saline, the trichomonads will not die and will not stain, forming a pink area around them, making it easier to identify the white protozoa. Alternatively, a drop of 1600x acridine orange solution can be added to the fresh sample, and observed under a fluorescence microscope to see the trichomonads with a pale yellow-green fluorescence, which is very beautiful. The detection rate of direct microscopy is extremely high.
(2) Smear staining method
Spread the secretion on a glass slide, wait for it to dry naturally, and then stain it with different stains, such as Gram staining, Wright staining, Giemsa staining, PAS staining, and Leishman staining. This method not only allows us to see the shape and content of the trichomonads but also enables us to see other microorganisms present in the vagina at the same time. Acridine orange staining and fluorescence microscopy can also be used.
(3) Culture method
Add vaginal secretions or urethral secretions to the culture medium, incubate in a 37℃ incubator for 48 hours, inoculate once every 72 hours, take one drop of the mixed culture solution for smearing, and then examine under a microscope after staining.
(4) Immunological methods
Detect the specific antigens of Trichomonas vaginalis. Common immunological methods include fluorescence antibody test, ELISA, latex agglutination test, etc., which have a higher positive rate than smear method, but are generally not used for clinical examination.
In summary, the above content describes the examination methods for Trichomonas vaginalis, which can help women better diagnose whether they have Trichomonas vaginalis. Once women find they have Trichomonas vaginalis, both partners should be treated, and the cleanliness of the vulva should be maintained.